Dermocosmetics & skin histology
Atlantic Bone Screen has been developing specific expertise in skin histology for the past 10 years. Staining and immuno-staining (IHC and IF) can be performed on paraffin and frozen samples and specific protocols can be developed under request or transferred from your Lab to ours.
Expertise on skin tissue
- On human natural or reconstructed skin samples
- On biopsies samples
- On all layers of the skin
Skin research Axis
- Inflammation and pathology
- Barrier and function
- Pigmentation
- Vascularization
- Aging
- Wound healing
- Dermal filler
Staining & immunostaining
- On paraffin or frozen samples
- +75 protocols (see examples below and catalog under request)
- Development and validation of new protocols under request
- Protocol transfer
Image acquisition
- Fields acquisition and reconstruction of composites
- Transmitted light and epifluorescence microscopy
- Slides digitalization (Nanozoomer)
- Confocal microscopy
Image analysis
- Cell quantification
- Tissue segmentation
- Tissue shape and size analysis
- Quantification of IHC/IF (e.g. Marker Detection, Quantification of Staining Intensity)
- Vascular analysis (quantification of vessels)
- Melanin Analysis
Non-exhaustive list of available staining and markers:
| α-Actin | Collagen VII | Elastin | Integrin α6 | MAGP-1 | Sirius Red |
| β-Catenin | Collagen XII | Fibrillin | Involucrin | Masson Trichrome | SKALP |
| CD31 | Cytokeratin 10 | Filaggrin | KANK-4 | Orcein | SUN-1 |
| CD44 | Cytokeratin 14 | F. Masson | Keratin 2, 31 | PAS | TGM-1 |
| Collagen I | Decorin | HE/HES | Keratin 35,71… | Perlecan | Vimentin |
| Collagen IV | Desmocollin | Heparan Sulf. | Ki67 | PGC-1α | |
| Collagen III | Desmoglein | Herovici | Laminin-5 | Procollagen I | under request… |