Check if you compound has a pro-inflammatory effect or an anti-inflammatory effect on macrophages M0 and M1
Skin explants
Assess the effect of your compounds on skin explants
Prior to testing compounds or formulations on actual human patients in the context of clinical research in cosmetics or dermatology, human skin explants are now the reference protocols to initially test candidates and get robust evidence of their non-toxicity and efficacy. This method is now the most valuable solution as an excellent alternative to animal testing.
Skin explants are adapted to evaluate toxicity, efficacy, and biocompatibility of your products.
Skin explants culture
Skin explant biopsies from healthy donors (from abdominal, face or breast) are managed according to ethical rules and quality procedures and are processed by our technicians using inserts:
• Culture fromJ1 to J7
• Treatment by topical application, intradermal injection or medium stimulation
• Daily medium renewal
Supernatants and skin explants are collected and analysed in our labs to evaluate the toxicity and/or the efficacy of the tested conditions. Many markers of skin pigmentation, hydratation, vascularization and inflammation can be explored for objectivation and comparison of products/formulation.
Gene & protein expression
A lot of parameters can be analysed by RT-qPCR or ELISA to evaluate intercellular communication, extracellular matrix components, dermo-epidermal junction, epidermal differentiation…
Histological processing & analysis
75 stainings & immunostainings, IHC & IF, and protocol development (see our catalog) for global structure or specific markers observations as epidermal differentiation, epidermal cohesion and intercellular exchanges, synthesis and expression of extracellular matrix components, dermal epidermal junction proteins, pigmentation…
Request our Human skin histology catalog >
Imaging analysis & quantification using IA
Once generated, histological slides are scanned and analysed bymanual/automated methods. Other imaging analyses are also available and can be adapted to match your
needs: area measurement, vessel and cells quantification, epidermal thickness, fluorescence intensity…
Expert services for histology natural skin or 3D printed samples: frozen and paraffin, IHC and IF, more than 75 validated markers and development under request
Thanks to current capabilities and expertise, our Team can develop specific custom model for in vitro assessment of your compounds and biomaterials