Assess the biodistribution or PK/PD of your compounds on healthy animals

Biodistribution and pharmacokinetic/pharmacodynamic (PK/PD) studies in rodents in the preclinical phase are essential for assessing the behaviour of drug candidates in vivo, optimising doses and predicting clinical effects.

Biodistribution studies are essential for evaluating how compounds spread throughout the body and identifying target and non-target organs. These analyses focus on key organs involved in metabolism, excretion, and pharmacological effects, including the liver, kidneys, lungs, brain, spleen, and reproductive and endocrine organs. The selection of organs for study depends on the compound’s mechanism of action and administration route.

The process involves collecting blood and relevant organs at various time points after administration to track distribution changes. Compound concentrations in tissues are measured using biochemical analyses, while histology and immunohistochemistry techniques allow for visualization and quantification of the product at the cellular level in tissue samples.

These studies provide crucial insights into a compound’s behavior in the body, helping to optimize drug development and ensure targeted therapeutic effects.

Pharmacokinetic studies are essential for understanding drug exposure following several doses of your compound either as single or repeated administration

We propose different type of blood sampling techniques, carefully selected based on volume requirements, sampling frequency, and animal welfare considerations. The goal is to obtain high-quality pharmacokinetic data while minimizing stress and discomfort for the animals. One effective method involves using implanted devices like vascular catheters, which allow for repeated sampling with reduced handling and stress.

Key pharmacokinetic parameters will be determined by analyzing blood samples collected at specific intervals following drug administration, including the maximum concentration (Cmax), time to reach maximum concentration (Tmax), half-life (t1/2), and area under the curve (AUC).

Pharmacodynamic studies play a crucial role in drug development and optimization exploring how a drug interacts with its target in the body, providing valuable insights into its effectiveness and safety profile. By examining the biochemical, physiological, and molecular effects of yout test compounds on target organs, specific biomarkers could be analyze in various biological samples such as serum, plasma, urine, synovial fluid, …. Additionally, these studies assess the drugs binding to its receptor and investigate the relationship between the administered dose and the observed response, helping to determine the optimal dosage.

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